ABSTRACT
Viral infection induces numerous tripartite motif (TRIM) proteins to control antiviral immune signaling and viral replication. Particularly, SPRY-containing TRIM proteins are found only in vertebrates and they control target protein degradation by their RING-finger and SPRY domains, and proper cytoplasmic localization. To understand TRIM30 function, we analyzed its localization pattern and putative roles of its RING-finger and SPRY domains. We found that TRIM30 is located in actin-mediated cytoplasmic bodies and produces colocalized ubiquitin chains in SPRY domain- and RING-finger domain-dependent ways that are degraded by autophagy and the proteasome. These results suggest a TRIM protein-dependent degradation mechanism by cytoplasmic body formation with actin networks.
Subject(s)
Animals , Mice , Amino Acid Sequence , Autophagy , Cell Line , Inclusion Bodies/metabolism , Intracellular Signaling Peptides and Proteins/chemistry , Molecular Sequence Data , Polyubiquitin/metabolism , Proteasome Endopeptidase Complex/metabolism , Protein Interaction Domains and Motifs , Protein Transport , Proteolysis , RING Finger DomainsABSTRACT
PURPOSE: We report clinical features and viral studies on 153 children with aseptic meningitis in the Pusan area in 1996 and compare the results of this study with those of other reports in 1990 and 1993. METHODS: One hundred fifty-three children with aseptic meningitis who have been admitted to the Department of Pediatrics, St. Benedict Hospital between July and October 1996 were included. We described age and sex distribution, monthly distribution, clinical manifestations, laboratory data and complication. RESULTS: The male to female ratio was 2.32:1 and the most age was 4.21 years. It occurred mostly in July, August and October. It was later than the peak incidence between May and July in 1990 and 1993. The main symptoms were fever, headache and vomiting in this order. Rash was mostly maculopapular form and frequent in those younger than 4 years-old. No specific results were not found in peripheral blood and CSF studies. We failed to isolate the causative agent in the viral culture, but Echovirus 9 was suspected as a causative agent by its clinical features and other similar reports. The duration of clinical symptoms in the early diagnostic group was significantly shorter that in late diagnostic group. CONCLUSIONS: Aseptic meningitis was prevalent in children in the Pusan area, during the summer of 1996. It was frequent in male and the mean age was 4.21 years. It occurred mostly in July, August and October. Peak incidence was later than in 1990 and 1993. The main symptoms: fever, headache, vomiting and rash were observed frequently.